This is an archived course. A more recent version may be available at ocw.mit.edu.

Calendar

NK = Dr. Natalie Kuldell

BE = Prof. Bevin Engelward

DE = Prof. Drew Endy

AB = Prof. Angela Belcher

SESĀ # LABS LECTURERS
1 Orientation NK
Module 1: DNA Engineering
2 DNA Engineering by PCR BE
3 Clean and Cut DNA BE
4 Agarose Gel Electrophoresis BE
5 DNA Ligation and Bacterial Transformation BE
6 Examine Candidate Clones BE
7 Restriction Map, Article Discussion/Tissue Culture BE
8 Lipofection BE
9 FACS Analysis BE
Module 2: Protein Engineering
10 Tools for Protein Engineering NK
11 Assessing Beta-galactosidase NK
12 Purifying Beta-galactosidase NK
13 Student Presentations NK
Module 3: Systems Engineering
14 Tools for Systems Engineering DE
15 Basic Bacterial Photography: Black and White DE
16 Advanced Bacterial Photography: 2 Color DE
17 Device Characterization DE
18 Measuring DNA, RNA, Protein DE
19 RT-PCR Data Analysis DE
Module 4: Bio-material Engineering
20 Screening Library AB
21 Optimizing Panning AB
22 Rescreening Gold Binders AB
23 PCR of Gold Binding Candidates AB
24 Student Presentations AB
25 Analyze Sequence Data AB