This is an archived course. A more recent version may be available at ocw.mit.edu.

Assignments

Except where noted in the table below, all assignments are due in the next class session.

SES # LABS ASSIGNMENTS
1 Orientation  
Module 1: DNA Engineering
2 DNA Engineering by PCR (PDF)
3 Clean and Cut DNA (PDF)
4 Agarose Gel Electrophoresis (PDF)
5 DNA Ligation and Bacterial Transformation (PDF)
6 Examine Candidate Clones (PDF)
7 Restriction Map, Article Discussion/Tissue Culture (PDF)
8 Lipofection (PDF)
9 FACS Analysis (PDF)
Module 2: Protein Engineering
10 Tools for Protein Engineering (PDF)
11 Assessing Beta-galactosidase (PDF)
12 Purifying Beta-galactosidase (PDF)
13 Student Presentations (PDF)
Module 3: Systems Engineering
14 Tools for Systems Engineering (PDF)
15 Basic Bacterial Photography: Black and White (PDF)
16 Advanced Bacterial Photography: 2 Color (PDF)
17 Device Characterization (PDF)
18 Measuring DNA, RNA, Protein (PDF)
19 RT-PCR Data Analysis  
Module 4: Bio-material Engineering
20 Screening Library (PDF)
21 Optimizing Panning (PDF)
22 Rescreening Gold Binders (PDF)
23 PCR of Gold Binding Candidates (PDF)
24 Student Presentations  
25 Analyze Sequence Data  

Lab Reports

Guidelines and Lecture Materials on Scientific Writing

Guidelines for Writing a Lab Report (PDF)

Scientific Rhetoric Overview (PDF)

Writing the Introduction Section (PDF)

Writing the Results and Methods Sections (PDF)

Oral Presentations

Guidelines and Lecture Materials on Giving Oral Presentations

Guidelines for Giving Oral Presentations (PDF)

Presenting Basics (PDF)

Presenting with a Partner (PDF)