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Reverse genetic approaches reveal gene redundancy in Arabidopsis anthers
(Massachusetts Institute of Technology, 2020)
When aquatic plants migrated to land 500 million years ago, they were met with harsh conditions associated with terrestrial life, such as dry air, high radiance light, and high effective gravity. Early plants of this time ...
Interactions between an integrative and conjugative element and its bacterial host
(Massachusetts Institute of Technology, 2021)
Conjugative elements are mobile genetic elements that can transfer from a donor bacterium to a recipient via an element-encoded type IV secretion system. Integrative and conjugative elements (ICEs) are an abundant class ...
Separase cleaves the kinetochore protein Meikin to direct the meiosis I/II transition
(Massachusetts Institute of Technology, 2021)
To generate haploid gametes, meiotic cells must undergo two consecutive rounds of chromosome segregation without an intervening gap phase. Importantly, because homologous chromosomes are segregated in meiosis I, but sister ...
Cell-Intrinsic and cell-extrinsic resistance to classical chemotherapies
(Massachusetts Institute of Technology, 2020)
Resistance is a constant presence in chemotherapy treatment. The very action of these therapeutic compounds causes resistance to these drugs. There are two modes of resistance to chemotherapy: the cancer cells themselves ...
Discovery and characterization of stable introns in yeast
(Massachusetts Institute of Technology, 2018)
Spliceosomal introns are a defining feature of eukaryotes; they are present in all known eukaryotic genomes, absent from all known non-eukaryotic genomes, and their accurate removal is essential for mRNA maturation. Although ...
Modulation of lineage-specific cell differentiation by long non-coding RNAs
(Massachusetts Institute of Technology, 2015)
Mammalian genomes comprise thousands of non-protein-coding genes. These can produce small non-coding RNAs (such as rRNAs and tRNAs), as well as long non-coding RNAs (lncRNAs), which are >200nt and resemble mRNAs in their ...
A GNAQ/11-driven zebrafish cancer model identifies MITF and YAP as key determinants for uveal melanoma
(Massachusetts Institute of Technology, 2019)
Uveal melanoma (UM) is a cancer of eye melanocytes that has a particularly poor prognosis and a dearth of treatment options. In contrast to cutaneous melanoma (CM), which is driven by oncogenic mutation of BRAF or NRAS, ...
Functional analyses of mitotic microtubule-binding complexes
(Massachusetts Institute of Technology, 2018)
Mitosis is the process by which a single cell divides to form two identical daughter cells. Each daughter cell must inherit a full complement of the genetic material. Thus, a critical aspect of mitosis is the faithful ...
Organization and regulation of outer kinetochore assembly
(Massachusetts Institute of Technology, 2015)
The kinetochore provides the connection between chromosomes and spindle microtubules. Defining the molecular architecture of the core kinetochore components is critical for understanding the mechanisms by which the kinetochore ...
Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions
(Massachusetts Institute of Technology, 2014)
The Drosophila neuromuscular junction (NMJ) is capable of rapidly budding new presynaptic varicosities over the course of minutes in response to elevated neuronal activity. Using live imaging of synaptic growth, we ...