dc.contributor.advisor | Peter T. C. So. | en_US |
dc.contributor.author | Kim, Yang-Hyo. | en_US |
dc.contributor.other | Massachusetts Institute of Technology. Department of Mechanical Engineering. | en_US |
dc.date.accessioned | 2019-07-19T19:36:40Z | |
dc.date.available | 2019-07-19T19:36:40Z | |
dc.date.copyright | 2018 | en_US |
dc.date.issued | 2019 | en_US |
dc.identifier.uri | https://hdl.handle.net/1721.1/121846 | |
dc.description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2019 | en_US |
dc.description | Cataloged from PDF version of thesis. | en_US |
dc.description | Includes bibliographical references. | en_US |
dc.description.abstract | The optical resolution of microscopy is limited by the wave-like characteristic of the light. There are many recent advances in overcoming this diffraction limited resolution, but mostly focused on fluorescent imaging. Furthermore, there are few non-fluorescence wide-field super-resolution techniques that can fully utilize the applicable laser power to optimize imaging speed. Structured illumination microscopy is a super-resolution method that relies on patterned excitation. This thesis has presented novel applications of structured illumination microscopy to surface plasmon resonance fluorescence and pump-probe scattering imaging. First, structured illumination microscopy was introduced to surface plasmon resonance fluorescence imaging for high signal-to-noise and high resolution. Secondly, a theoretical framework for three-dimensional wide-field pump-probe structured illumination microscopy has been developed to increase the lateral resolution and enable depth sectioning. Further, structured illumination wide-field photothermal digital phase microscopy is proposed as a high throughput, high sensitivity super-resolution imaging tool to diagnose ovarian cancer. Finally, I have derived the exact analytical solution to the heat conduction problem in which a sphere absorbs temporally modulated laser beam for photothermal microscopy. The proposed method also has a great potential to be applied to other pump-probe modalities such as transient absorption and stimulated Raman scattering. | en_US |
dc.description.sponsorship | Funding sources and sponsors: National Institutes of Health (9P41EB015871-26A1, 5R01NS051320, 4R44EB012415, and 1R01HL121386-OlAl), National Science Foundation (CBET-09395 11), Hamamatsu Corporation, Singapore-Massachusetts Institute of Technology Alliance for Research and Technology (SMART) Center, BioSystems and Micromechanics (BioSyM), and Samsung Scholarship | en_US |
dc.description.statementofresponsibility | by Yang-Hyo Kim. | en_US |
dc.format.extent | 112 pages | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Massachusetts Institute of Technology | en_US |
dc.rights | MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. | en_US |
dc.rights.uri | http://dspace.mit.edu/handle/1721.1/7582 | en_US |
dc.subject | Mechanical Engineering. | en_US |
dc.title | Wide-field structured illumination microscopy for fluorescence and pump-probe imaging | en_US |
dc.type | Thesis | en_US |
dc.description.degree | Ph. D. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Mechanical Engineering | en_US |
dc.identifier.oclc | 1102058096 | en_US |
dc.description.collection | Ph.D. Massachusetts Institute of Technology, Department of Mechanical Engineering | en_US |
dspace.imported | 2019-07-19T19:36:08Z | en_US |
mit.thesis.degree | Doctoral | en_US |
mit.thesis.department | MechE | en_US |