This is an archived course. A more recent version may be available at ocw.mit.edu.

Recitations

Laboratory sessions begin with a short recitation reviewing basic guidelines and principles for the assigned experiments of that day.

A sessionwise listing of downloadable notes appear in the table below, courtesy of and used with permission from the following contributors: Genetics module notes by Eric Sullivan; Protein Biochemistry module notes by Anonymous; Recombinant DNA Methods notes by Laura Anne Lowery; Recombinant DNA Methods Overview notes by Michael Lawrence; Development notes by Fang Xie.

SES # TOPICS NOTES
Introduction and Training Lab
2 Training Lab  
3 Training Lab (cont.)  
Module 1: Genetics

Genetics Module Overview (PDF)

Mutagenesis Strategy and Central Dogma (PDF)
5 Genetics Lab 1 (PDF)
6 Genetics Lab 1 (cont.)  
8 Genetics Lab 2 (PDF)
9 Genetics Lab 2 (cont.) (PDF)
11 Genetics Lab 3 (PDF)
12 Genetics Lab 3 (cont.)  
14 Genetics Lab 4 (PDF)
15 Genetics Lab 4 (cont.)  
17 Genetics Lab 5 (PDF)
19 Genetics Lab 5 (cont.)  
20 Genetics Lab 6 (PDF)
Module 2: Protein Biochemistry
22 Genetics Lab 6 (cont.)  
23 Genetics Lab 7

Protein Biochemistry Lab 1
Hames, B. D., and D. Rickwood, eds. Gel Electrophoresis of Proteins: A Practical Approach. 3rd ed. New York: Oxford University Press, 1998. ISBN: 9780199636402. (Practical Approach Series)

Protein Biochemistry Lab 1 Notes (PDF)
25 Genetics Lab 7 (cont.)

Protein Biochemistry Lab 1 (cont.)
 
26 Protein Biochemistry Lab 2 (PDF)
28 Protein Biochemistry Lab 2 (cont.)  
30 Protein Biochemistry Lab 3 (PDF - 1.5 MB)
31 Protein Biochemistry Lab 3 (cont.)  
32 Protein Biochemistry Lab 4 (PDF)
34 Protein Biochemistry Lab 4 (cont.)  
35 Protein Biochemistry Lab 5 (PDF - 2.2 MB)
37 Protein Biochemistry Lab 5 (cont.)  
38 Protein Biochemistry Lab 6 (PDF - 2.1 MB)
Module 3: Recombinant DNA Methods

Recombinant DNA Methods Overview (PDF)
40 Protein Biochemistry Lab 6 (cont.)  
43 Recombinant DNA Methods Lab 1 (PDF)
44 Recombinant DNA Methods Lab 1 (cont.)  
46 Recombinant DNA Methods Lab 2 (PDF)
48 Recombinant DNA Methods Lab 2 (cont.)  
49 Recombinant DNA Methods Lab 3 (PDF - 1.2 MB)
50 Recombinant DNA Methods Lab 3 (cont.)  
52 Recombinant DNA Methods Lab 4 (PDF)
53 Recombinant DNA Methods Lab 4 (cont.)  
55 Recombinant DNA Methods Lab 5 (PDF)
56 Recombinant DNA Methods Lab 5 (cont.)  
Module 4: Development
58 Recombinant DNA Methods Lab 6 (PDF )
60 Recombinant DNA Methods Lab 6 (cont.)  
62 Recombinant DNA Methods Lab 7

Development Lab 1

Recombinant DNA Methods Lab 7 Notes (PDF)

Development Lab 1 Notes (PDF)

63 Recombinant DNA Methods Lab 7 (cont.)

Development Lab 1 (cont.)
 
64 Development Lab 2 (PDF)
65 Development Lab 2 (cont.)  
67 Development Lab 3 (PDF)
69 Development Lab 3 (cont.)  
71 Development Lab 4 (PDF)
72 Development Lab 4 (cont.)  
74 Development Lab 5 (PDF)
75 Development Lab 5 (cont.)