dc.contributor.author | Tian, Y. | |
dc.contributor.author | Ravi, P. | |
dc.contributor.author | Bromberg, Lev | |
dc.contributor.author | Hatton, T. Alan | |
dc.contributor.author | Tam, K. C. | |
dc.date.accessioned | 2004-12-16T06:15:15Z | |
dc.date.available | 2004-12-16T06:15:15Z | |
dc.date.issued | 2005-01 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/7498 | |
dc.description.abstract | Poly(acrylic acid) (PAA) was grafted onto both termini of Pluronic F87 (PEO₆₇-PPO₃₉-PEO₆₇) via atom transfer radical polymerization to produce a novel muco-adhesive block copolymer PAA₈₀-b-F₈₇-b-PAA₈₀. It was observed that PAA₈₀-F₈₇-PAA₈₀ forms stable complexes with weakly basic anti-cancer drug, Doxorubicin. Thermodynamic changes due to the drug binding to the copolymer were assessed at different pH by isothermal titration calorimetry (ITC). The formation of the polymer/drug complexes was studied by turbidimetric titration and dynamic light scattering. Doxorubicin and PAA-b-F87-b-PAA block copolymer are found to interact strongly in aqueous solution via non-covalent interactions over a wide pH range. At pH>4.35, drug binding is due to electrostatic interactions. Hydrogen-bond also plays a role in the stabilization of the PAA₈₀-F₈₇-PAA₈₀/DOX complex. At pH 7.4 (α=0.8), the size and stability of polymer/drug complex depend strongly on the doxorubicin concentration. When CDOX <0.13mM, the PAA₈₀-F₈₇-PAA₈₀ copolymer forms stable inter-chain complexes with DOX (110 ~ 150 nm). When CDOX >0.13mM, as suggested by the light scattering result, the reorganization of the polymer/drug complex is believed to occur. With further addition of DOX (CDOX >0.34mM), sharp increase in the turbidity indicates the formation of large aggregates, followed by phase separation. The onset of a sharp enthalpy increase corresponds to the formation of a stoichiometric complex. | en |
dc.description.sponsorship | Singapore-MIT Alliance (SMA) | en |
dc.format.extent | 26912 bytes | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | |
dc.relation.ispartofseries | Molecular Engineering of Biological and Chemical Systems (MEBCS); | |
dc.subject | Poly(acrylic acid) | en |
dc.subject | Pluronic F87 | en |
dc.subject | atom transfer radical polymerization | en |
dc.subject | ATRP | en |
dc.subject | muco-adhesive block copolymers | en |
dc.subject | CDOX | en |
dc.subject | Doxorubicin | en |
dc.title | Synthesis and Aggregation Behavior of Pluronic F87/Poly(acrylic acid) Block Copolymer with Doxorubicin | en |
dc.type | Article | en |