Requests for Comments (RFCs): Recent submissions

Now showing items 58-60 of 74

    • Units for Promoter Measurement in Mammalian Cells 

      Velten, Lars; Iwamoto, Nao; Hiller, Corinna; Uckelmann, Hannah; Zhu, Chenchen; e.a. (2009-10-21)
      The purpose of this RFC is to provide units for the characterization of promoter strength for use in mammalian cells. RMPU is mRNA based and directly proportional to PoPS, whereas REU is protein based and not proportional ...

    • BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction 

      DiCarlo, James; Boeke, Jef (2009-10-07)
      The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. ...

    • BBF RFC 21: BglBricks Assembly Standard 

      Anderson, J Christopher; Dueber, John E; Leguia, Mariana; Wu, Gabriel C; Goler, Jonathan A; e.a. (2009-09-18)
      The BglBricks standard has been developed as an alternative to the original XbaI/SpeI standard primarily to provide a solution to the issue of generating translational-fusion parts. When joined together in a standard ...