Requests for Comments (RFCs): Recent submissions

Now showing items 13-15 of 74

    • HiCT: High Throughput Protocols For CPE Cloning And Transformation 

      Beer, Ralf; Christiansen, Tania; Herbst, Konrad; Ignatiadis, Nikolaos; Kats, Ilia; e.a. (2013-10-04)
      The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...

    • GenBrick – A Rapid Multi-Part Assembly Method for BioBricks 

      Gasiūnaitė, Lina; Lewicka, Aleksandra; Pashkuleva, Hristiana; Villanueva, Hugo; Thornton, Harry; e.a. (2013-10-04)
      This BioBricks Foundation Request for Comments (BBF RFC) describes a novel approach, GenBrick, for the rapid assembly of multiple BioBrick RFC10-compatible parts in a single reaction, with completely flexible part order, ...

    • The AutoAnnotator - Standardized annotation of protein-encoding BioBricks 

      Wolf, Christopher; Albrecht, Florian; Brüggenthies, Johanna; Brunner, Andreas; Ciccone, Rosario; e.a. (2013-10-04)
      For protein-encoding BioBricks a large number of useful parameters, such as the amino acid sequence or the molecular weight, can easily be computed. Furthermore alignments against databases and predictions of various ...