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HiCT: High Throughput Protocols For CPE Cloning And Transformation
(2013-10-04)
The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...
Reusable rapid assembly of genetic parts for Neurospora crassa
(2011-10-17)
This BBF RFC provides a method in which standardized parts can be easily created for Neurospora crassa.
PCR - Ligation Assembly Standard for BioBrick Parts
(2011-12-15)
This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This ...
The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly
(2012-11-05)
BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ...
Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units
(2012-10-11)
BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note ...
"BioSandwich", a homology-based assembly method using a library of standard parts
(2011-10-17)
This Request for Comments (RFC) describes a strategy for using homology-based assembly methods to assemble parts from a library in any order.
GenBrick – A Rapid Multi-Part Assembly Method for BioBricks
(2013-10-04)
This BioBricks Foundation Request for Comments (BBF RFC) describes a novel approach, GenBrick, for the rapid assembly of multiple BioBrick RFC10-compatible parts in a single reaction, with completely flexible part order, ...