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Conversion of Freiburg (Fusion) Biobricks to the Silver (BioFusion) format
(2009-03-30)
This Request for Comments (RFC) describes a strategy for converting Biobricks
from the Freiburg (aka Fusion) format to the Silver lab (aka BioFusion)
format.
Instructions to BBF RFC Authors
(OpenWetWare, 2008-11-10)
This BioBricks Foundation Request for Comments (BBF RFC) provides in-
formation about the preparation and submission of BBF RFCs to The Bio-
Bricks Foundation (BBF).
Standard for Synthesis of Customized Peptides by Non-Ribosomal Peptide Synthetases
(2013-10-04)
The purpose of this RFC is to introduce a standardized framework for the engineering of customizable non-ribosomal peptide synthetases (NRPS) and their application for in vivo and in vitro synthesis of short non-ribosomal ...
Open Sequence Initiative: a part submission standard to complement modern DNA assembly techniques
(2013-10-06)
The discipline of synthetic biology emphasizes the application of engineering principles such as standardization, abstraction, modularity, and rational design to complex biological systems. The archetypical example of such ...
The Measurement of rho‐independent Transcription Terminator Efficiency
(2012-10-11)
The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of terminators in bacteria. Because we have characterized the performance of terminator in E. coli and used a simple ...
The AutoAnnotator - Standardized annotation of protein-encoding BioBricks
(2013-10-04)
For protein-encoding BioBricks a large number of useful parameters, such as the amino acid sequence or the molecular weight, can easily be computed. Furthermore alignments against databases and predictions of various ...
HiCT: High Throughput Protocols For CPE Cloning And Transformation
(2013-10-04)
The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...
BBF RFC 94: Type IIS Assembly for Bacterial Transcriptional Units: A Standardized Assembly Method for Building Bacterial Transcriptional Units Using the Type IIS Restriction Enzymes BsaI and BbsI
(2015-08-28)
This RFC94 describes an assembly standard based on the Type IIS restriction enzymes BsaI and BbsI (also called BpiI). This assembly standard is based upon the Modular Cloning (MoClo) assembly strategy, which was introduced ...
Provisional BioBrick Language (PoBoL)
(2009-05-15)
This BioBricks Foundation Request for Comments (BBF RFC) describes a semantic markup language for publishing and sharing information about BioBricks on the World Wide Web.
This BBF RFC includes the recommendation for the ...
Reusable rapid assembly of genetic parts for Neurospora crassa
(2011-10-17)
This BBF RFC provides a method in which standardized parts can be easily created for Neurospora crassa.