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BBF RFC 94: Type IIS Assembly for Bacterial Transcriptional Units: A Standardized Assembly Method for Building Bacterial Transcriptional Units Using the Type IIS Restriction Enzymes BsaI and BbsI
(2015-08-28)
This RFC94 describes an assembly standard based on the Type IIS restriction enzymes BsaI and BbsI (also called BpiI). This assembly standard is based upon the Modular Cloning (MoClo) assembly strategy, which was introduced ...
BBF RFC 104: BrickClip – rapid assembly of multiple RFC10 BioBricks
(2015-03-17)
This BioBrick Foundation Request for Comments (BBF RFC 104) describes a new approach to multiple part DNA assembly – BrickClip, which does not require use of any restriction enzymes, nor cloning of the parts to specific ...
BBF RFC 105: The Intein standard - a universal way to modify proteins after translation
(2015-03-17)
This Request for Comments (RFC) proposes a new standard that allows for easy and flexible cloning of intein constructs and thus makes this technology accessible to the synthetic biology community.
BBF RFC 107: Copyright and Licensing of BBF RFCs
(2015-08-28)
In this BioBricks Foundation Request for Comments (BBF RFC), we update and clarify the copyright and licensing notice of BBF RFC documents.
BBF RFC 106: A Standard Type IIS Syntax for Plants
(2015-03-17)
Here we define a standard syntax for assembling standard parts for expression in plant cells, extensible to all other eukaryotes. Variations of the Type IIS mediated cloning method known as Golden Gate Cloning, most notably ...
BBF RFC 108: Synthetic Biology Open Language (SBOL) Version 2.0.0
(2015-09-07)
The Synthetic Biology Open Language (SBOL) has been developed as a standard to support the specification and exchange of biological design information in synthetic biology, filling a need not satisfied by other pre-existing ...