Search
Now showing items 1-5 of 5
The Measurement of rho‐independent Transcription Terminator Efficiency
(2012-10-11)
The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of terminators in bacteria. Because we have characterized the performance of terminator in E. coli and used a simple ...
Synthetic Biology Open Language (SBOL) Version 1.1.0
(2012-10-11)
In this BioBricks Foundation Request for Comments (BBF RFC), we specify the Synthetic Biology
Open Language (SBOL) Version 1.1.0 to enable the electronic exchange of information
describing DNA components used in synthetic ...
The minimum information for a qualified BioBrick
(2012-10-11)
Since the information of many existing BioBricks is incomplete, thus the usage of the BioBricks will be affected. It is necessary to standardize the minimum information required for a qualified BioBrick. Furthermore this ...
The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly
(2012-11-05)
BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ...
Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units
(2012-10-11)
BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note ...