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Standard Biological Part Automatic Modeling Database Language (MoDeL)
(2010-11-09)
This BioBricks Foundation Request for Comments (BBF RFC) describes the Standard Biological Part
Automatic Modeling Database Language (MoDeL). MoDeL provides a language and syntax standard
for automatic modeling databases ...
Quantitative measurement of mammalian cell invasion by bacteria using flow cytometry
(2010-10-31)
Purpose of this RFC is to provide method of measurement of bacterial invasion into mammalian cells. Also this RFC defines Percent of INvasion (PIN) unit.
Fast multiple gene fragment ligation method based on homologous recombination
(2010-10-31)
With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at ...
Open licensing of BioBrick parts
(2010-12-05)
This document provides recommendations for licensing of community-created biological parts, especially in BioBrick standard.
Standard for the Electronic Distribution of SBOLv Diagrams
(2010-12-05)
A common method for publicly distributing design diagrams on the Web would enhance understanding of the mechanisms and intended function of synthetic DNA constructs. We aim to improve the usefulness of the depicted design ...
General updating scheme for the design and construction of assembly vectors that are compatible with BBF RFC 10 and BBF RFC 28
(2010-12-05)
BBF RFC 75 intends to link the BBF RFC 28-based combinatorial multi-part assembly method to BBF RFC 10. Its aim is to easily redesign an expression vector to make it compatible to BBF RFC 10 for the construction of fusion ...
Abbreviated BioBrick Prefix and Suffix for More Efficient Primer Design
(2010-10-31)
This Request for Comments (RFC) modifies the assembly standard for biological parts proposed in BBF RFC 10 by removing the NotI restriction site from the BioBrick Prefix and Suffix.
miTuner - a kit for microRNA based gene expression tuning in mammalian cells
(2010-12-05)
The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ...
miMeasure – a standard for miRNA binding site characterization in mammalian cells
(2010-12-05)
This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...
Recombination Based Part Assembly
(2010-12-05)
Here we propose a new recombination-based assembly standard, optimized to allow for efficient cloning of mammalian vectors.