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miTuner - a kit for microRNA based gene expression tuning in mammalian cells
(2010-12-05)
The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ...
miMeasure – a standard for miRNA binding site characterization in mammalian cells
(2010-12-05)
This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...
Cloning Standard for Mammalian BioBrick Parts and Devices
(2009-10-21)
To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.
Units for Promoter Measurement in Mammalian Cells
(2009-10-21)
The purpose of this RFC is to provide units for the characterization of promoter strength for use in mammalian cells. RMPU is mRNA based and
directly proportional to PoPS, whereas REU is protein based and not proportional ...
Design of Specific Mammalian Promoters by in silico Prediction
(2009-10-30)
The purpose of this RFC is to provide a) a method for the design of rational
synthetic promoter sequences based on a statistical analysis about
the spatial preference of transcription factor binding sites in human promoter ...