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Standard for Synthesis of Customized Peptides by Non-Ribosomal Peptide Synthetases
(2013-10-04)
The purpose of this RFC is to introduce a standardized framework for the engineering of customizable non-ribosomal peptide synthetases (NRPS) and their application for in vivo and in vitro synthesis of short non-ribosomal ...
HiCT: High Throughput Protocols For CPE Cloning And Transformation
(2013-10-04)
The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...
miTuner - a kit for microRNA based gene expression tuning in mammalian cells
(2010-12-05)
The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ...
miMeasure – a standard for miRNA binding site characterization in mammalian cells
(2010-12-05)
This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...
RA-PCR, a method for the generation of randomized promoter libraries
(2009-10-21)
The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of ...
BBF RFC 105: The Intein standard - a universal way to modify proteins after translation
(2015-03-17)
This Request for Comments (RFC) proposes a new standard that allows for easy and flexible cloning of intein constructs and thus makes this technology accessible to the synthetic biology community.
Cloning Standard for Mammalian BioBrick Parts and Devices
(2009-10-21)
To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.
Units for Promoter Measurement in Mammalian Cells
(2009-10-21)
The purpose of this RFC is to provide units for the characterization of promoter strength for use in mammalian cells. RMPU is mRNA based and
directly proportional to PoPS, whereas REU is protein based and not proportional ...
Design of Specific Mammalian Promoters by in silico Prediction
(2009-10-30)
The purpose of this RFC is to provide a) a method for the design of rational
synthetic promoter sequences based on a statistical analysis about
the spatial preference of transcription factor binding sites in human promoter ...