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Standard for Synthesis of Customized Peptides by Non-Ribosomal Peptide Synthetases
(2013-10-04)
The purpose of this RFC is to introduce a standardized framework for the engineering of customizable non-ribosomal peptide synthetases (NRPS) and their application for in vivo and in vitro synthesis of short non-ribosomal ...
HiCT: High Throughput Protocols For CPE Cloning And Transformation
(2013-10-04)
The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...
RA-PCR, a method for the generation of randomized promoter libraries
(2009-10-21)
The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of ...
Cloning Standard for Mammalian BioBrick Parts and Devices
(2009-10-21)
To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.