Browsing Requests for Comments (RFCs) by Title
Now showing items 42-61 of 74
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The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly
(2012-11-05)BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ... -
HiCT: High Throughput Protocols For CPE Cloning And Transformation
(2013-10-04)The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ... -
How to Build Kinetic Models of BioBricks
(2009-10-21)This BioBricks Foundation Request for Comments (BBF RFC) provides instructions on how to automatically generate a model of any BioBrick sequence. These models can be used in computer simulations of the dynamic behavior of ... -
Instructions to BBF RFC Authors
(OpenWetWare, 2008-11-10)This BioBricks Foundation Request for Comments (BBF RFC) provides in- formation about the preparation and submission of BBF RFCs to The Bio- Bricks Foundation (BBF). -
Large-Scale Peptide Modification on BioBrick Proteins
(2009-10-30)With the established BioBrick Assembly standards, fusion proteins with diversified functions are easy to manipulate. However, we realized that the existing BBF RFC do not clearly defining the standard for peptide display ... -
Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR
(2010-12-05)The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, ... -
The Measurement of rho‐independent Transcription Terminator Efficiency
(2012-10-11)The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of terminators in bacteria. Because we have characterized the performance of terminator in E. coli and used a simple ... -
miMeasure – a standard for miRNA binding site characterization in mammalian cells
(2010-12-05)This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ... -
The minimum information for a qualified BioBrick
(2012-10-11)Since the information of many existing BioBricks is incomplete, thus the usage of the BioBricks will be affected. It is necessary to standardize the minimum information required for a qualified BioBrick. Furthermore this ... -
miTuner - a kit for microRNA based gene expression tuning in mammalian cells
(2010-12-05)The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ... -
Naming of standards of physical composition of BioBrick parts
(2009-04-17)This BioBricks Foundation Request for Comments (BBF RFC) proposes a naming convention for standards of physical composition of BioBrick parts in which assembly standards are named according to the BBF RFC that specifies ... -
A New Standard to Connect BioBrick Parts for Precise Extraction of an Enzyme Digestion Product
(2010-12-05)This BioBricks Foundation Request for Comments (BBF RFC) introduces a new standard to connect BioBrick parts using BglI site. -
Novel Normalization Standard using Fluorescence
(2010-12-05)The Biotec_Dresden Team 2010 developed an approach where two fluorescent proteins are simultaneously expressed. The fact that one reporter, in our case RFP, is constitutively expressed allows to monitor cell growth. Secondly, ... -
Open licensing of BioBrick parts
(2010-12-05)This document provides recommendations for licensing of community-created biological parts, especially in BioBrick standard. -
Open Sequence Initiative: a part submission standard to complement modern DNA assembly techniques
(2013-10-06)The discipline of synthetic biology emphasizes the application of engineering principles such as standardization, abstraction, modularity, and rational design to complex biological systems. The archetypical example of such ... -
PCR - Ligation Assembly Standard for BioBrick Parts
(2011-12-15)This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This ... -
Promoter and Coding Sequence Considerations for Caenorhabditis elegans and Other Eukaryotes
(2010-11-07)Our primary aim is to provide a standard format for protein-coding BioBricks intended for eukaryotes, especially in the nematode worm Caenorhabditis elegans, so that future teams will be able to correctly take advantage ... -
Proposed Conceptual Guidelines for the Design of a BioBrick Graphical Language & an Example
(2009-03-12)As we update the current BioBrick symbols we have an opportunity to look at the end users of a BioBrick Graphical language and develop design guidelines so that the new symbols best suit their needs. This document explores ... -
Provisional BioBrick Language (PoBoL)
(2009-05-15)This BioBricks Foundation Request for Comments (BBF RFC) describes a semantic markup language for publishing and sharing information about BioBricks on the World Wide Web. This BBF RFC includes the recommendation for the ... -
Quantitative measurement of mammalian cell invasion by bacteria using flow cytometry
(2010-10-31)Purpose of this RFC is to provide method of measurement of bacterial invasion into mammalian cells. Also this RFC defines Percent of INvasion (PIN) unit.