Browsing Requests for Comments (RFCs) by Title

Now showing items 32-51 of 74

    • Detailed Information Standard 

      Culviner, Peter; Pantalone, Nathaniel; Sagstetter, Mary; Sandock, Sarah; Vrana, Justin; e.a. (2010-12-05)
      The purpose of this standard is to increase the required information that must accompany any parts submitted to the Registry of Standard Biological Parts. This will give users of the parts better assurance of their ...

    • Documentation of BioBrick parts 

      Slomp, Arend; Ekkers, David (2010-12-05)
      Purpose of this RFC is to improve the information supplied by BioBrick part vendors. Reason to have this RFC is that there would be much more information available which can help improve the search time of the user. ...

    • Draft Standard for Biobrick BB-2 Biological Parts 

      Knight, Tom (OpenWetWare, 2008-11-19)
      This standard defines the required sequence properties for a Biobrick(R) BB-2 standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards.

    • Draft Standard for Biobrick Biological Parts 

      Knight, Tom (OpenWetWare, 2007-05-03)
      This standard defines the required sequence properties for a Biobrick(tm) standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards. ...

    • DTU Synthetic Promoter Library Standard 

      Fortuna, Patrick; Trolle, Thomas; Borch, Martin Malthe; Haugaard, Anastasiya; Kjaergaard, Maya Friis; e.a. (2010-12-04)
      The purpose of this RFC is to outline a method for generating a BioBrick compatible Synthetic Promoter Library (SPL) within bacteria in order to fine-tune the expression of BioBrick parts and devices.

    • Fast multiple gene fragment ligation method based on homologous recombination 

      Wang, Ruiyan; Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
      With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at ...

    • Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII 

      Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
      With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at ...

    • Fusion Protein (Freiburg) Biobrick assembly standard 

      Grünberg, Raik; Arndt, Katja; Müller, Kristian (2009-04-18)
      This Request for Comments (RFC) describes an extension to the original BioBrick assembly standard (BBF RFC 10). The Fusion Assembly strategy described here is fully backwards compatible with RFC 10 and supports the ...

    • GenBrick – A Rapid Multi-Part Assembly Method for BioBricks 

      Gasiūnaitė, Lina; Lewicka, Aleksandra; Pashkuleva, Hristiana; Villanueva, Hugo; Thornton, Harry; e.a. (2013-10-04)
      This BioBricks Foundation Request for Comments (BBF RFC) describes a novel approach, GenBrick, for the rapid assembly of multiple BioBrick RFC10-compatible parts in a single reaction, with completely flexible part order, ...

    • General updating scheme for the design and construction of assembly vectors that are compatible with BBF RFC 10 and BBF RFC 28 

      Billerbeck, Sonja; Zwicky, Katharina; Lang, Moritz; Pestalozzi, Luzius; Rosenberger, George; e.a. (2010-12-05)
      BBF RFC 75 intends to link the BBF RFC 28-based combinatorial multi-part assembly method to BBF RFC 10. Its aim is to easily redesign an expression vector to make it compatible to BBF RFC 10 for the construction of fusion ...

    • The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly 

      Pauthenier, Cyrille; El-Sayyed, Hafez; Rostain, William; Cerisy, Tristan; Lopez, Carolina Gallo; e.a. (2012-11-05)
      BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ...

    • HiCT: High Throughput Protocols For CPE Cloning And Transformation 

      Beer, Ralf; Christiansen, Tania; Herbst, Konrad; Ignatiadis, Nikolaos; Kats, Ilia; e.a. (2013-10-04)
      The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...

    • How to Build Kinetic Models of BioBricks 

      Weeding, Emma; Houle, Jason; Swiniarski, Ben; Smadbeck, Patrick; Lindblad, Kristen; e.a. (2009-10-21)
      This BioBricks Foundation Request for Comments (BBF RFC) provides instructions on how to automatically generate a model of any BioBrick sequence. These models can be used in computer simulations of the dynamic behavior of ...

    • Instructions to BBF RFC Authors 

      Tarjan, Daniel; Sleight, Sean; Shetty, Reshma; Ridgway, Doug; Rettberg, Randy; e.a. (OpenWetWare, 2008-11-10)
      This BioBricks Foundation Request for Comments (BBF RFC) provides in- formation about the preparation and submission of BBF RFCs to The Bio- Bricks Foundation (BBF).

    • Large-Scale Peptide Modification on BioBrick Proteins 

      Tian, Feng; Chen, GuoQiang; Wang, Zhao (2009-10-30)
      With the established BioBrick Assembly standards, fusion proteins with diversified functions are easy to manipulate. However, we realized that the existing BBF RFC do not clearly defining the standard for peptide display ...

    • Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR 

      Olchowik, Anna; Lower, Michal; Moreno, Cherry; Pankowski, Jaroslaw (2010-12-05)
      The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, ...

    • The Measurement of rho‐independent Transcription Terminator Efficiency 

      He, Jiankui; Jing, Shui; Zhang, Mengshi; Liu, Xianggeng; Chen, Yao; e.a. (2012-10-11)
      The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of terminators in bacteria. Because we have characterized the performance of terminator in E. coli and used a simple ...

    • miMeasure – a standard for miRNA binding site characterization in mammalian cells 

      Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; e.a. (2010-12-05)
      This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...

    • The minimum information for a qualified BioBrick 

      Zhou, Mubing; Zhang, Junqiu; He, Jiankui (2012-10-11)
      Since the information of many existing BioBricks is incomplete, thus the usage of the BioBricks will be affected. It is necessary to standardize the minimum information required for a qualified BioBrick. Furthermore this ...

    • miTuner - a kit for microRNA based gene expression tuning in mammalian cells 

      Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; e.a. (2010-12-05)
      The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ...