Browsing Requests for Comments (RFCs) by Title

Now showing items 21-40 of 74

    • BBF RFC 94: Type IIS Assembly for Bacterial Transcriptional Units: A Standardized Assembly Method for Building Bacterial Transcriptional Units Using the Type IIS Restriction Enzymes BsaI and BbsI 

      Haddock, Traci L.; Densmore, Douglas M.; Appleton, Evan; Carr, Swati; Iverson, Sonya; e.a. (2015-08-28)
      This RFC94 describes an assembly standard based on the Type IIS restriction enzymes BsaI and BbsI (also called BpiI). This assembly standard is based upon the Modular Cloning (MoClo) assembly strategy, which was introduced ...

    • BBF RFC97: Genetic Circuit Standard 1.0 

      Wang, Lingjue; Zhu, Xiaodan; Li, Hanrun; He, Huiyu; Ni, Zhuangdian; e.a. (2013-10-29)
      Current, there is well adopted documentation of biobricks. The Registry of Standard Biology Part set the standard documentation for biobrick. However, there is no standard way to document a genetic circuit, what information ...

    • "BioSandwich", a homology-based assembly method using a library of standard parts 

      Abbas, Yassen; Crossman, Allan; Fletcher, Eugene; French, Chris; Gibson, Clare; e.a. (2011-10-17)
      This Request for Comments (RFC) describes a strategy for using homology-based assembly methods to assemble parts from a library in any order.

    • Bioscaffold-Linker 

      Mina, Petros; Savery, Nigel (2009-10-21)
      Protein fusions are currently not supported by most assembly standards. This assembly standard aims to provide the basis of a solution to a biobrick-friendly protein fusion mechanism that supports the current favorite ...

    • Building Protein Domain Based Composite Biobricks for Mammalian Expression Systems 

      Balint, Balint L; Keret, Ophir; Brazda, Peter; Demeny, Mate; 2010 Debrecen-Hungary, iGEM (2010-12-05)
      The purpose of this RFC is to describe a method that allows the design of protein domain based parts, starting with gene centered information and translate these informations into BBF RFC 25 compatible part. The method is ...

    • Cloning Standard for Mammalian BioBrick Parts and Devices 

      Bartoschek, Michael; Mugahid, Douaa; Rademacher, Anne; Meyer, Hannah; Velten, Lars; e.a. (2009-10-21)
      To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.

    • Constraint Relaxation of RFC 10 for Assembling Standard Biological Parts 

      Che, Austin (2009-03-30)
      This Request for Comments (RFC) modifies the assembly standard for biological parts proposed in BBF RFC 10 by replacing PstI with SbfI and dropping other sequence constraints. This change relaxes the constraints on the ...

    • Construction a RBS library with different translational activity 

      Lee, Hsiao-Ching (2010-12-05)
      The first step in programming and controlling cell behavior is to establish a library of well-defined components, a.k.a. "biobricks", that serve as the building blocks of artificial gene networks. The main challenge in ...

    • Conversion of Freiburg (Fusion) Biobricks to the Silver (BioFusion) format 

      Grunberg, Raik (2009-03-30)
      This Request for Comments (RFC) describes a strategy for converting Biobricks from the Freiburg (aka Fusion) format to the Silver lab (aka BioFusion) format.

    • Creating native registry functions to accommodate mutant libraries 

      Xu, Kenneth O.; Walters, Eric M. (2011-12-29)
      The current registry system accommodates normal parts fairly well but has difficulty when adding mutant libraries. If all mutant offspring were added as new parts, the registry would be comprehensive but the parts registry ...

    • Design of Specific Mammalian Promoters by in silico Prediction 

      Heinemann, Tim; Kramer, Stephen; Velten, Lars; Kranz, Anna-Lena; Bauer, Tobias; e.a. (2009-10-30)
      The purpose of this RFC is to provide a) a method for the design of rational synthetic promoter sequences based on a statistical analysis about the spatial preference of transcription factor binding sites in human promoter ...

    • Detailed Information Standard 

      Culviner, Peter; Pantalone, Nathaniel; Sagstetter, Mary; Sandock, Sarah; Vrana, Justin; e.a. (2010-12-05)
      The purpose of this standard is to increase the required information that must accompany any parts submitted to the Registry of Standard Biological Parts. This will give users of the parts better assurance of their ...

    • Documentation of BioBrick parts 

      Slomp, Arend; Ekkers, David (2010-12-05)
      Purpose of this RFC is to improve the information supplied by BioBrick part vendors. Reason to have this RFC is that there would be much more information available which can help improve the search time of the user. ...

    • Draft Standard for Biobrick BB-2 Biological Parts 

      Knight, Tom (OpenWetWare, 2008-11-19)
      This standard defines the required sequence properties for a Biobrick(R) BB-2 standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards.

    • Draft Standard for Biobrick Biological Parts 

      Knight, Tom (OpenWetWare, 2007-05-03)
      This standard defines the required sequence properties for a Biobrick(tm) standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards. ...

    • DTU Synthetic Promoter Library Standard 

      Fortuna, Patrick; Trolle, Thomas; Borch, Martin Malthe; Haugaard, Anastasiya; Kjaergaard, Maya Friis; e.a. (2010-12-04)
      The purpose of this RFC is to outline a method for generating a BioBrick compatible Synthetic Promoter Library (SPL) within bacteria in order to fine-tune the expression of BioBrick parts and devices.

    • Fast multiple gene fragment ligation method based on homologous recombination 

      Wang, Ruiyan; Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
      With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at ...

    • Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII 

      Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
      With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at ...

    • Fusion Protein (Freiburg) Biobrick assembly standard 

      Grünberg, Raik; Arndt, Katja; Müller, Kristian (2009-04-18)
      This Request for Comments (RFC) describes an extension to the original BioBrick assembly standard (BBF RFC 10). The Fusion Assembly strategy described here is fully backwards compatible with RFC 10 and supports the ...

    • GenBrick – A Rapid Multi-Part Assembly Method for BioBricks 

      Gasiūnaitė, Lina; Lewicka, Aleksandra; Pashkuleva, Hristiana; Villanueva, Hugo; Thornton, Harry; e.a. (2013-10-04)
      This BioBricks Foundation Request for Comments (BBF RFC) describes a novel approach, GenBrick, for the rapid assembly of multiple BioBrick RFC10-compatible parts in a single reaction, with completely flexible part order, ...