Browsing Requests for Comments (RFCs) by Author "Cai, Yizhi"

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    • BBF RFC 104: BrickClip – rapid assembly of multiple RFC10 BioBricks 

      Trubitsyna, Maryia; Chan, Karen; Cai, Yizhi; Elfick, Alistair; French, Chris (2015-03-17)
      This BioBrick Foundation Request for Comments (BBF RFC 104) describes a new approach to multiple part DNA assembly – BrickClip, which does not require use of any restriction enzymes, nor cloning of the parts to specific ...

    • Synthetic Biology Open Language (SBOL) Version 1.1.0 

      Galdzicki, Michal; Wilson, Mandy; Rodriguez, Cesar A.; Pocock, Matthew R.; Oberortner, Ernst; e.a. (2012-10-11)
      In this BioBricks Foundation Request for Comments (BBF RFC), we specify the Synthetic Biology Open Language (SBOL) Version 1.1.0 to enable the electronic exchange of information describing DNA components used in synthetic ...

    • Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units 

      Boeke, Jef; Mitchell, Leslie; Cai, Yizhi; Stracquadanio, Giovanni; Chuang, James; e.a. (2012-10-11)
      BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note ...