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The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly
(2012-11-05)
BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ...
Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units
(2012-10-11)
BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note ...