Simultaneous whole‐animal 3D imaging of neuronal activity using light‐field microscopy
Author(s)Prevedel, Robert; Yoon, Young-Gyu; Hoffman, Maximilian; Pak, Nikita; Wetzstein, Gordon; Kato, Saul; Schrödel, Tina; Raskar, Ramesh; Zimmer, Manuel; Boyden, Edward S.; Vaziri, Alipasha; ... Show more Show less
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High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ~700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
Notes: Robert Prevedel*, Young‐Gyu Yoon*, Maximilian Hoffmann, Nikita Pak, Gordon Wetzstein, Saul Kato, Tina Schrödel, Ramesh Raskar, Manuel Zimmer, Edward S Boyden** & Alipasha Vaziri** (* equal contributions, ** co-corresponding authors)
Center for Brains, Minds and Machines (CBMM)
CBMM Memo Series;016
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