LOCUS       pCH1497-ASD1anno        9964 bp    DNA     circular     22-AUG-2006
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|379863731|
COMMENT     VNTDBDATE|419435310|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCH1497-ASD1annotated|
COMMENT     VNTAUTHORNAME|Barry Canton|
COMMENT     VNTAUTHOREML|bcanton@mit.edu|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "Tetracycline resistance gene" 4 0 0 8706 9896 0 
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COMMENT                          "Created: 07/12/05 01:42PM" 1) 
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COMMENT                          "length: 9964 bp" 1) 
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COMMENT                                        "I found this sequence by comparing the p15a ori from pACYC184 to the sequence of pCH1497-ASD1" 
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COMMENT                                        "This includes all the upstream sequence of the plasmid that matches the rrnB operon.  There are 188 bp upstream from the start of the rrnB gene.  That means that I have the first 13bp of the P2 promoter" 
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FEATURES             Location/Qualifiers
     rRNA            2118..3846
                     /vntifkey="58"
                     /label=rrnB\16s\rRNA
                     /note="This includes all the upstream sequence of the plasmid that matches the rrnB operon.  There are 188 bp upstream from the start of the rrnB gene.  That means that I have the first 13bp of the P2 promoter"
     promoter        882..2091
                     /vntifkey="30"
                     /label=pBAD
     rRNA            7283..7342
                     /vntifkey="58"
                     /label=rrnB\5s\rRNA
     rRNA            4288..7191
                     /vntifkey="58"
                     /label=rrlB\23S\rRNA
     misc_feature    7591..7591
                     /vntifkey="21"
                     /label=End\of\homology\to\rrnB\operon
     CDS             8706..9896
                     /vntifkey="4"
                     /label=Tetracycline\resistance\gene
     rep_origin      7708..8618
                     /vntifkey="33"
                     /label=p15a\ori\(partial)
                     /note="I found this sequence by comparing the p15a ori from pACYC184 to the sequence of pCH1497-ASD1"
     promoter        2118..2131
                     /vntifkey="30"
                     /label=Partial\P2\promoter
     misc_feature    9904..9950
                     /vntifkey="21"
                     /label=sequence\from\pACYC184
     primer_bind     3934..3952
                     /vntifkey="28"
                     /label=pCH1497PCRR
     misc_feature    3838..3842
                     /vntifkey="21"
                     /label=ASD
     primer_bind     2112..2112
                     /vntifkey="28"
                     /label=start\of\pCH1410F\sequence\data
     misc_feature    4690..4690
                     /vntifkey="21"
                     /label=Start\of\SacIF\sequence\data
     primer_bind     complement(4583..4583)
                     /vntifkey="28"
                     /label=Start\of\SacIR\sequence\data
     misc_feature    complement(7675..7675)
                     /vntifkey="21"
                     /label=Start\of\pCH1410R\sequence\data
     primer_bind     1297..1316
                     /vntifkey="28"
                     /label=G00700\(fwd)
     primer_bind     complement(1660..1679)
                     /vntifkey="28"
                     /label=G00701\(rev)
BASE COUNT     2462 a      2477 c      2816 g      2209 t 
ORIGIN
        1 agaactgtga atgcgcaaac caacccttgg tcatggcgct cataaatctg gtatacttac 
       61 ctttacacca tggggctgat tctggattcg acgggatttg cgaaacccaa ggtgcatgcc 
      121 gaggggcggt tggcctcgta aaaagccgca aaaaatagtc gcaaacgacc atcaccacca 
      181 tcatcacgat taataaccat cttagagccc tctctcccta gcctccgctc ttaggacggg 
      241 gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc cctgcctggg gttgaagcgt 
      301 taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc cgcagctggc aagcgaatgt 
      361 aaagactgac taagcatgta gtaccgagga tgtaggaatt tcggacgcgg gttcaactcc 
      421 cgccagctcc accaagaatt ctccatcggt aagagcccgc acggcgcaag ccctgcgggc 
      481 ttttttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt 
      541 tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat 
      601 gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt 
      661 ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac 
      721 cgccggacat cagcgctagc aaaaaagcct gctcgttgag caggcttttc gaatttggcg 
      781 cgccccatcg atgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta 
      841 tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct 
      901 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat 
      961 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg 
     1021 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg 
     1081 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc 
     1141 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg 
     1201 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta 
     1261 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa 
     1321 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc 
     1381 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc 
     1441 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc 
     1501 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt 
     1561 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg 
     1621 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc 
     1681 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga 
     1741 tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt 
     1801 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa 
     1861 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa 
     1921 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc 
     1981 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc 
     2041 tttttatcgc aactctctac tgtttctcca tacccgtttt tttgggctag caggaggaat 
     2101 tccatatagc tcctcgagta ttatgcacac cccgcgccgc tgagaaaaag cgaagcggca 
     2161 ctgctcttta acaatttatc agacaatctg tgtgggcact cgaagatacg gattcttaac 
     2221 gtcgcaagac gaaaaatgaa taccaagtct caagagtgaa cacgtaattc attacgaagt 
     2281 ttaattcttt gagcgtcaaa cttttaaatt gaagagtttg atcatggctc agattgaacg 
     2341 ctggcggcag gcctaacaca tgcaagtcga acggtaacag gaagaagctt gcttctttgc 
     2401 tgacgagtgg cggacgggtg agtaatgtct gggaaactgc ctgatggagg gggataacta 
     2461 ctggaaacgg tagctaatac cgcataacgt cgcaagacca aagaggggga ccttcgggcc 
     2521 tcttgccatc ggatgtgccc agatgggatt agctagtagg tggggtaacg gctcacctag 
     2581 gcgacgatcc ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc 
     2641 agactcctac gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc 
     2701 catgccgcgt gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg 
     2761 agtaaagtta atacctttgc tcattgacgt tacccgcaga agaagcaccg gctaactccg 
     2821 tgccagcagc cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag 
     2881 cgcacgcagg cggtttgtta agtcagatgt gaaatccccg ggctcaacct gggaactgca 
     2941 tctgatactg gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa 
     3001 atgcgtagag atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg 
     3061 ctcaggtgcg aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa 
     3121 acgatgtcga cttggaggtt gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt 
     3181 cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca 
     3241 caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc tggtcttgac 
     3301 atccacggaa gttttcagag atgagaatgt gccttcggga accgtgagac aggtgctgca 
     3361 tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt cccgcaacga gcgcaaccct 
     3421 tatcctttgt tgccagcggt ccggccggga actcaaagga gactgccagt gataaactgg 
     3481 aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg accagggcta cacacgtgct 
     3541 acaatggcgc atacaaagag aagcgacctc gcgagagcaa gcggacctca taaagtgcgt 
     3601 cgtagtccgg attggagtct gcaactcgac tccatgaagt cggaatcgct agtaatcgtg 
     3661 gatcagaatg ccacggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccatg 
     3721 ggagtgggtt gcaaaagaag taggtagctt aaccttcggg agggcgctta ccactttgtg 
     3781 attcatgact ggggtgaagt cgtaacaagg taaccgtagg ggaacctgcg gttggatcac 
     3841 acacttacct taaagaagcg tactttgtag tgctcacaca gattgtctga tagaaagtga 
     3901 aaagcaaggc gtttacgcgt tgggagtgag gctgaagaga ataaggccgt tcgctttcta 
     3961 ttaatgaaag ctcaccctac acgaaaatat cacgcaacgc gtgataagca attttcgtgt 
     4021 ccccttcgtc tagaggccca ggacaccgcc ctttcacggc ggtaacaggg gttcgaatcc 
     4081 cctaggggac gccacttgct ggtttgtgag tgaaagtcgc cgaccttaat atctcaaaac 
     4141 tcatcttcgg gtgatgtttg agatatttgc tctttaaaaa tctggatcaa gctgaaaatt 
     4201 gaaacactga acaacgagag ttgttcgtga gtctctcaaa ttttcgcaac acgatgatga 
     4261 atcgaaagaa acatcttcgg gttgtgaggt taagcgacta agcgtacacg gtggatgccc 
     4321 tggcagtcag aggcgatgaa ggacgtgcta atctgcgata agcgtcggta aggtgatatg 
     4381 aaccgttata accggcgatt tccgaatggg gaaacccagt gtgtttcgac acactatcat 
     4441 taactgaatc cataggttaa tgaggcgaac cgggggaact gaaacatcta agtaccccga 
     4501 ggaaaagaaa tcaaccgaga ttcccccagt agcggcgagc gaacggggag cagcccagag 
     4561 cctgaatcag tgtgtgtgtt agtggaagcg tctggaaagg cgcgcgatac agggtgacag 
     4621 ccccgtacac aaaaatgcac atgctgtgag ctcgatgagt agggcgggac acgtggtatc 
     4681 ctgtctgaat atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga 
     4741 accagtaccg tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga 
     4801 aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg tgactgcgta ccttttgtat 
     4861 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa 
     4921 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca 
     4981 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa 
     5041 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct 
     5101 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc 
     5161 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta 
     5221 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg 
     5281 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc 
     5341 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt 
     5401 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacgct 
     5461 tatgcgttgt tgggtagggg agcgttctgt aagcctgcga aggtgtgctg tgaggcatgc 
     5521 tggaggtatc agaagtgcga atgctgacat aagtaacgat aaagcgggtg aaaagcccgc 
     5581 tcgccggaag accaagggtt cctgtccaac gttaatcggg gcagggtgag tcgaccccta 
     5641 aggcgaggcc gaaaggcgta gtcgatggga aacaggttaa tattcctgta cttggtgtta 
     5701 ctgcgaaggg gggacggaga aggctatgtt ggccgggcga cggttgtccc ggtttaagcg 
     5761 tgtaggctgg ttttccaggc aaatccggaa aatcaaggct gaggcgtgat gacgaggcac 
     5821 tacggtgctg aagcaacaaa tgccctgctt ccaggaaaag cctctaagca tcaggtaaca 
     5881 tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag aataccaagg cgcttgagag 
     5941 aactcgggtg aaggaactag gcaaaatggt gccgtaactt cgggagaagg cacgctgata 
     6001 tgtaggtgag gtccctcgcg gatggagctg aaatcagtcg aagataccag ctggctgcaa 
     6061 ctgtttatta aaaacacagc actgtgcaaa cacgaaagtg gacgtatacg gtgtgacgcc 
     6121 tgcccggtgc cggaaggtta attgatgggg ttagcgcaag cgaagctctt gatcgaagcc 
     6181 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa 
     6241 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga 
     6301 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc 
     6361 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggctttg 
     6421 aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta atgtttgatg 
     6481 ttctaacgtt gacccgtaat ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg 
     6541 cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg gtcggacatc 
     6601 aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg cgagcaggtg 
     6661 cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct caacggataa 
     6721 aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac ggcggtgttt 
     6781 ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag ggtatggctg 
     6841 ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag ttcggtccct 
     6901 atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag gaccggagtg 
     6961 gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc taaatgcgga 
     7021 agagataagt gctgaaagca tctaagcacg aaacttgccc cgagatgagt tctccctgac 
     7081 cctttaaggg tcctgaagga acgttgaaga cgacgacgtt gataggccgg gtgtgtaagc 
     7141 gcagcgatgc gttgagctaa ccggtactaa tgaaccgtga ggcttaacct tacaacgccg 
     7201 aagctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag 
     7261 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc 
     7321 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc 
     7381 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 
     7441 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag 
     7501 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa 
     7561 ctgccaggca tcaaattaag cagaaggcca tcctgcagga ttcgaacctg cggcccacga 
     7621 cttagaaggt cgttgctcta tccaactgag ctaagggcgc aatgcggggc gcatcttact 
     7681 gcgcagatac gccctcgtca atcccttgct agcggagtgt atactggctt actatgttgg 
     7741 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg 
     7801 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc 
     7861 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa 
     7921 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata 
     7981 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc 
     8041 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg 
     8101 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca 
     8161 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc 
     8221 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa 
     8281 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt 
     8341 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc 
     8401 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg 
     8461 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga 
     8521 agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa 
     8581 tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt tgacagctta 
     8641 tcatcgataa gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac 
     8701 cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg 
     8761 taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg 
     8821 acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat 
     8881 gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt 
     8941 cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc 
     9001 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct 
     9061 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt 
     9121 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct 
     9181 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct 
     9241 tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc 
     9301 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct 
     9361 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg 
     9421 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc 
     9481 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg 
     9541 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc 
     9601 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg 
     9661 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat 
     9721 cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt 
     9781 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc 
     9841 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg 
     9901 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt 
     9961 gcgg 
//
